abstract：:The articular cartilage is frequently destroyed in rheumatic diseases. Among the various responsible factors, enzymes and especially collagenases play a leading role. Their action explains the collagen degradation linked with cartilage destruction. The author recalls the origin, mechanism of action of the articular co...

journal_title：Annales de biologie clinique

authors： Mitrovic D

更新日期：1986-01-01 00:00:00

abstract：:Serum level monitoring of drugs allows an optimal drug therapy, because it can take into account individual pharmacokinetic (and metabolic) variations. Some special conditions in drugs and in patients are highly suggestive of drug monitoring. These include mainly physiological but also pharmacological situations and a...

journal_title：Annales de biologie clinique

authors： Riant P,Albengres E,Barre J,Tillement JP

更新日期：1986-01-01 00:00:00

abstract：:In vitro fertilization (IVF) and embryo transfer (ET) appear to constitute a revolution in the reproductive sciences rather than merely a new technique in the treatment of sterility. Principle of IVF: IVF accomplishes in vitro the process than normally occurs in the oviduct between the ovulation of oocyte II and embry...

journal_title：Annales de biologie clinique

authors： Hédon B,Marès P,Humeau C,Garcia G,Flandre O,Galand B,Arnal F,Cristol P,Deschamps F

更新日期：1985-01-01 00:00:00

abstract：:Patients with renal insufficiency who are hemodialyzed 3 times a week either through a cuprophane membrane or through a polyacrylonitrile (PAN) membrane have the same transcortin and cortisol levels as patients with renal insufficiency who are not dialyzed. Moreover, hemodialysis did not influence acutely the latter t...

journal_title：Annales de biologie clinique

authors： De Moor P,Faict D,Verberckmoes R

更新日期：1985-01-01 00:00:00

abstract：:The analyzers have been defined as follows: the analyzers are multiparametric (at least 5 parameters available simultaneously), selective, operating patient by patient, list price on May 1, 1984 below 1,000,000 francs, and distributed in France after January 1, 1981. This definition applies in particular to systems al...

journal_title：Annales de biologie clinique

authors： Grafmeyer D,Vassault A,Feuillu A,Naudin C

更新日期：1985-01-01 00:00:00

abstract：:Haloperidol is extracted with hexane-isoamyl alcohol in the presence of two internal standards, chlorohaloperidol and spiroperidol. For chromatographic separation, a C18 reversed-phase column and a mobile phase of acetonitrile/methanol/phosphate buffer are used. Detection is by UV absorption spectrophotometry at 245 n...

journal_title：Annales de biologie clinique

authors： Hequet D,Jarry C,Rouquette C,Brachet-Liermain A

更新日期：1985-01-01 00:00:00

abstract：:Until now, the methods of analysis by luminescence have not developed greatly in clinical biology because of the instability of the reagents and the absence of a single protocol applicable to a sufficiently wide range of assays. For this reason, the application has been limited to several assays using a specific biolu...

journal_title：Annales de biologie clinique

authors： Nicolas JC,Terouanne B,Boussioux AM,Crastes de Paulet A

更新日期：1985-01-01 00:00:00

abstract：:The notion of risk indicator depends on the background of its application. The search for a better understanding of pathological processes implies a rigorous methodology in order to establish a link between the indicator and the risk. Today, the clinician often resorts the Bayes' theorem without taking in account the ...

journal_title：Annales de biologie clinique

authors：

更新日期：1985-01-01 00:00:00

abstract：:Three methods (rapid bioassay, enzyme immunoassay and fluoroimmunoassay) for the determination of amikacin level in serum are compared. The statistical analysis of the results show correlation coefficients equal to 0.967 (EMIT versus FIA), 0.970 (bioassay versus FIA) and 0.983 (bioassay versus EMIT). The three methods...

journal_title：Annales de biologie clinique

authors： Damien JM,Courcol RJ,Houdret N,Lhermitte M,Martin GR

更新日期：1984-01-01 00:00:00

abstract：:Con A stimulated lymphocytes from some patients with primary glomerular disease release in vitro a lymphokine, the vascular permeability factor (VPF). House dust sensitization was early reported among these patients. We therefore studied the action of this specific mitogen on VPF production in 58 patients with primary...

journal_title：Annales de biologie clinique

authors： Laurent J,Heslan JM,Branellec A,Lagrue G

更新日期：1984-01-01 00:00:00

abstract：:In the present investigation, the immunoglobulins G of human sera were studied after isoelectrofocusing using polyacrylamide flat bed gel. The heterogeneity of immunoglobulins was demonstrated in normal serum as well as in pathological situations. Isoelectrofocusing study was performed using the total serum or the imm...

journal_title：Annales de biologie clinique

authors： Chopin N,Lacour B,Thierry D,Faure A,Bailly M

更新日期：1983-01-01 00:00:00

abstract：:Using gas chromatography, we measured the concentrations of amino acids in a group of 80 selected subjects: we present the plasma values which we obtained, as well as those of other authors. Various comparisons were made, according to the age and/or sex of the subjects. We found that the plasma levels of valine, leuci...

journal_title：Annales de biologie clinique

authors： Cynober L,Blonde F,Nguyen Dinh F,Gerbet D,Giboudeau J

更新日期：1983-01-01 00:00:00

abstract：:Bacterial infectious pathology presents a series of new features which have been elucidated over the last few decades. These new aspects are derived from various factors: a development of our state of knowledge, a change in the ecology of a number of bacteria and a change in the receptiveness of patients. Modern infec...

journal_title：Annales de biologie clinique

authors： Wauters G

更新日期：1983-01-01 00:00:00

abstract：:This review of the immunological diagnosis of parasitic diseases defines the various indications, the means of collection and preparation, the various levels of specificity and the choice of parasitic antigen which should be used for immuno-diagnosis. The detection and assay of circulating antibodies relies on the tec...

journal_title：Annales de biologie clinique

authors： Carlier Y,Wery M

更新日期：1983-01-01 00:00:00

abstract：:Strictly aerobic Gram negative bacteria are found more and more often in human pathological specimens and in the environment. Amongst these bacteria, Flavobacterium poses problems of bacteriological diagnosis as their precise taxonomy is still recent. In this study, conducted on 321 strains of Flavobacterium from vari...

journal_title：Annales de biologie clinique

authors： Richard C,Monteil H

更新日期：1983-01-01 00:00:00

abstract：:Gas-liquid chromatography has been used directly on clinical specimens as a method for presumptive diagnosis of anaerobic infections. This technique proved to be valuable and reliable. Combined with a Gram stain, it offers the possibility to differentiate in less than 1 hour aerobic from anaerobic infections by means ...

journal_title：Annales de biologie clinique

authors： Hansen W,Labbé M,Pepersack F,Schoutens-Serruys E,Yourassowsky E

更新日期：1983-01-01 00:00:00

abstract：:We propose a double antibody radioimmunoassay for human prostatic acid phosphatase (PAP) in serum for diagnosis and management of prostatic adenocarcinoma under treatment. The antigen is purified from human prostatic fluid by a gel-filtration on Sephadex G 100 followed by affinity chromatography on Con A Sepharose. A ...

journal_title：Annales de biologie clinique

authors： Pradalier N,Canal P,Pujol A,Fregevu Y,Soula G

更新日期：1982-01-01 00:00:00

abstract：:Eighteen paraplegics with or without neurogenic osteo-arthropathy (POAN) were evaluated for variations in their urinary excretion levels of total (T), and free (L) hydroxyproline and its non-dialysable (ND) and dialysable (D) peptide fractions. Increases in urinary hydroxyproline, moderate rises in free hydroxyproline...

journal_title：Annales de biologie clinique

authors： Pilonchery G,Finck C,Minaire P,Messy P,Revol A

更新日期：1982-01-01 00:00:00

abstract：:The need for knowledge concerning foetal pulmonary maturity has led biochemists to develop a number of techniques based upon knowledge of amniotic phospholipids. Study of these phospholipids of foetal pulmonary origin is possible using biochemical and physical methods. The former include the L/S (Lecithin/sphingomyeli...

journal_title：Annales de biologie clinique

authors： Motta C

更新日期：1982-01-01 00:00:00

abstract：:There has been rapid progress in techniques for the detection of antinuclear antibodies. The methods most widely used at present are indirect immunofluorescence, Ouchterlony double immunodiffusion, passive haemagglutination and the Farr test. Antinuclear antibodies recognise several antigens. With a few exceptions, in...

journal_title：Annales de biologie clinique

authors： Abuaf N

更新日期：1982-01-01 00:00:00

abstract：:We report a turbidimetric procedure, using commercially available reagents, for the determination of plasma fibronectin with an unmodified centrifugal analyser. Fixed-time kinetic readings at 340 nm were taken at 60 and 720 s. A wide range of concentration (up to 1000 mg/l) can be measured. Precision and accuracy were...

journal_title：Annales de biologie clinique

authors： Hamel G,Toazara DJ,Mark J

更新日期：1982-01-01 00:00:00

abstract：:Insoluble elastin immobilised in Agar plates was used as substrate to quantitate the elastase inhibitory capacity of human sera. This technique was found valuable to evaluate the genetic deficiency of alpha-1-antitrypsin (Z phenotypes) and also the functional loss of the elastase inhibitory capacity in smokers. A sign...

journal_title：Annales de biologie clinique

authors： Bellon G,Hornebeck W,Lagrue G,Martin JP,Robert L

更新日期：1981-01-01 00:00:00

abstract：:Hemoglobin Alc is the result of non-enzymatic binding of a glucose molecule at the NH2 terminal of the beta chain. Assay of glycosylated hemoglobin is of great utility for the control efficacity of antidiabetic treatments, since such levels provide the clinician with an excellent index of glucose metabolism over the w...

journal_title：Annales de biologie clinique

authors： Wajcman H,Kitzis A

更新日期：1981-01-01 00:00:00

abstract：:A liquid chromatography procedure for routine determination of urinary estriol concentrations is described. After enzymic hydrolysis of a 1 ml sample, automatic extraction with chloroform/ethyl acetate, purification by chromatography on a disposable "minicolumn" of AG 1-X2, urinary estrogens are separated with normal ...

journal_title：Annales de biologie clinique

authors： Habrioux G

更新日期：1981-01-01 00:00:00

abstract：:Attention has been drawn in recent years to the diagnostic interest offered by increases in serum gamma GT in the detection of liver metastases and various cancers in man. Although this analysis is not specific and presents a few false positive and negative results, it seems however profitable to include it either in ...

journal_title：Annales de biologie clinique

authors： Braun JP,Soula G,Rico AG,Benard P,Burgat-Sacaze V

更新日期：1981-01-01 00:00:00

abstract：:Microsomal epoxide hydrolase (EC 3.3.2.3. ; formerly EC 4.2.1.63) catalyse the hydration of epoxides into trans dihydro-diols, easily excretable hydrophil compounds. It is an enzyme with very wide specificity for monosubstituted oxiranes, or cis disubstituted oxiranes. It is inactive on polysubstituted epoxides. The h...

journal_title：Annales de biologie clinique

authors： Dansette PM

更新日期：1980-01-01 00:00:00

abstract：:The recent development of technics of specific fixation using radio isotope-labelled tracer elements permits the demonstration of various types of receptors at the level of the lymphocyte membrane. Thus a new route is open in the understanding of the biochemical mechanisms controlling the physiology of the lymphocyte ...

journal_title：Annales de biologie clinique

authors： Arrang JM,Bidart JM

更新日期：1980-01-01 00:00:00

abstract：:An automatic continuous flow method is described for assay of plasmatic and urinary urea after hydrolysis by urease followed by NH4 assay with glutamic deshydrogenase in the same working solution. Precision and accuracy, comparison with chemical diacetylmonoxim method are studied. The interaction of endogenous NH4, th...

journal_title：Annales de biologie clinique

authors： Orsonneau JL,Meflah K,Cornu G,Bernard S

更新日期：1980-01-01 00:00:00

abstract：:The purpose of this study is to find, besides the determination of lactate in blood, a suitable and specific indicator of brain damage in newborn. Accordingly, we studied the total creatine kinase (CK) activity, by the method of Rosalki, and its subunit isoenzymes BB (CK BB) by fluorescence following electrophoresis o...

journal_title：Annales de biologie clinique

authors： Chopin N,Lavaud J,Voyer M,Bally M,Cloup M

更新日期：1980-01-01 00:00:00

abstract：:The authors propose a modification of the method of use of the API 20E system permitting more rapid identification of Enterobacteriaceae within six hours (3 hours preculture and 3 hours incubation on an API 20E plate) it was possible to identify correctly 67% of 192 strains studied at species level and 75.5% studied a...

journal_title：Annales de biologie clinique

authors： Pinon G,Loulergue J,Quentin R,Darchis JP,Prieur D,Vargues R

更新日期：1979-01-01 00:00:00

abstract：:The evidence for disturbances of tryptophan and 5-hydroxytryptamine in depression and for various mechanisms by which such disturbances could occur is discussed. Two recent relevant studies in the author's laboratory are described: a) non-esterified fatty acid and total and free tryptophan were determined in plasmas o...

journal_title：Annales de biologie clinique

authors： Curzon G

更新日期：1979-01-01 00:00:00

abstract：:The authors present the interest of electrosyneresis for diagnosis of purulent meningitis. Test material is a study about 120 cerebrospinal fluids. Electrosyneresis sometimes permits to identify the germ when microscopic examination and culture are negative or when microscopy is positive and the culture negative. Appl...

journal_title：Annales de biologie clinique

authors： Barege H,Rigal P,Morel A,Lemeland JF,Boiron H

更新日期：1979-01-01 00:00:00

abstract：:Diagnosis of type III hyperlipoproteinemia requires preparative ultracentrifugation in order to measure the cholesterol/triglyceride molar ratio into isolated d less than 1.006 lipoproteins. The authors describe an ultracentrifugation micromethod which needs 600 microliter of serum and can be completed within three ho...

journal_title：Annales de biologie clinique

authors： Moulin S,Dewailly P,Sezille G,Fruchart JC

更新日期：1979-01-01 00:00:00

abstract：:The erythrocyte bilirubin value is of great interest in neonate pathology. Its sudden rising reflects the overbinding capacity to plasmatic albumin. A colorimetric, simple and accurate micromethod with 2-hydrazino-benzothiazol as reagent leads to its quantitative determination. Hemolysis interference is removed. The a...

journal_title：Annales de biologie clinique

authors： Moreau-Clevede J,Pays M

更新日期：1979-01-01 00:00:00

abstract：:Hydrolysis of a solution of amylopectin by serum or urinary amylase leads to a reduction of the turbidity of this solution, observed at 340 nm. Adapted to centrifugal analysis, this method permits 15 estimations in 3 minutes with a serial reproducibility of 2.7 per cent, and a daily reproducibility of 6 per cent. The ...

journal_title：Annales de biologie clinique

authors： Houdret N,Porchet N,Lebas J,Douche MA,Degand P,Roussel P

更新日期：1979-01-01 00:00:00

abstract：:A rapid method of preparation of ornithine carbamyl transferase from rat liver is described. The enzyme preparation obtained is lacking in carbamyl phosphate synthetase activity. It may thus be used for the estimation of this enzyme on liver fragments obtained by needle biopsy of the liver in the diagnosis of neonatal...

journal_title：Annales de biologie clinique

authors： Rabier D,Cathelineau L,Kamoun P

更新日期：1978-01-01 00:00:00

abstract：:Bamifylline in therapeutic dosage may markedly disturb the determination of urinary 17-oxosteroids by Zimmermann's reaction, although the conditions of extraction and formation of this chromogenic substance are not always optimal for the metabolites of bamifylline. It is easy to suspect this interference as the chromo...

journal_title：Annales de biologie clinique

authors： Thévenet M,Maldonado J,Caillens H,Firmin C

更新日期：1978-01-01 00:00:00

abstract：:The relations between chromosomal diseases and mental retardation can be envisaged in three different ways: 1) Pharmacological approach shows a peculiar sensitivity of the cholinergic system in trisomic 21 children. A trouble in the manufacture of cholinergic mediator is plausible. 2) General biochemistry shows a slig...

journal_title：Annales de biologie clinique

authors： Lejeune J

更新日期：1978-01-01 00:00:00

abstract：:The development of enzymo-immunological technics and the synthesis of efficacious immuno-adsorbants has given rise to several immuno-enzymometric methods of which one, ELISA (enzyme-linked immunosorbent assay) has proved particularly practical and sensitive for the estimation of antibodies. ...

journal_title：Annales de biologie clinique

authors： Duvivier J

更新日期：1978-01-01 00:00:00

abstract：:The authors describe a method of fractionation of urinary 17-oxosteroids after purification of the urinary extract by Girard's T reagent. After solvolysis, enzyme hydrolysis and purification by Girard's T reagent, the 17-oxosteroids were estimated in the form of trimethyl silyl ethers by gas liquid chromatographye on ...

journal_title：Annales de biologie clinique

authors： Habrioux G

更新日期：1978-01-01 00:00:00